Chitosan activates defense responses and triterpenoid production in cell suspension cultures of Betula platyphylla Suk

摘要

Triterpenoid production and plant defense enzyme activity in suspension cultures ofBetula platyphylla?Suk. cells?treated with?chitosan?elicitor were investigated.Chitosan, at the optimal concentration of 100 mgL-1?enhanced triterpenoid production by 2.6-fold, with a maximum yield of 202.75 versus 78.72 mgL-1?of the untreated control cells. Furthermore, the activities of superoxide dismutase, phenylalanine ammonialyase, endochitinases and exochitinases?increased?by 1.6 ~ 3.0 fold. These results indicate that triterpenoid accumulation in?B. platyphylla?Suk. cell?are the consequence of a plant defense response?to chitosan elicitation.