Therapeutic Effects of Hydro-Alcoholic Extract of Clove Buds and Econocapus Erictus on Infected Wounds Healing in Dogs

摘要

The aim of this study was to evaluate the effect of two plants hydro-alcoholic (ethanol) extracts on wounds infected with resistance Staph. Aureus as ointments and in vitro, by comparing their with 1% of penicillin ointment as control positive. Serial dilutions used to determine the MIC and MBC of extracts and penicillin.Thirty two mature street dogs were divided into four equal groups (control, treated one, treated two and treated three). In control group (C)applied penicillin ointments on the infected wound topically, treated one group (T1) received 16mg/ml of C. erictus as ointment during 3 days after wound infected.while treated two group (T2) the infected wound treated with 6mg/ml of clove buds extract as ointment. Treated three group (T3) same procedure mention above but treated with combination ointment (C. erictus ointment and clove ointment) in ratio 1:1. The results revealed that MIC and MBC of C.erictus, clove buds and penicillin were (8mg/ml-16mg/ml, 5mg/ml-10mg/ml and 2μg/ml-4 μg/ml) respectively. The clinical parameters included temperature, respiratory rate , and heart rates showed no significances (p≤ 0.05) between groups. The scar present large quantitate in control group when compared with other group, the lesser scar formation was visible in T3 group. Histopathological Examinations was done by taken the skin biopsies on 15rd, and 30th, day post wound infection, the histopathological results revealed that the wound healing in T3 group faster and better than in the other groups and the epithelial cells more maturity and the skin taken same normal shape in T3 group comparing with other groups. In conclusion we are demonstrate that clove buds and C. erictus hydro-alcoholic extracts have valuable pharmacological properties include antibacterial, anti-inflammatory and anti-pyritic in vitro and on infected wound with resistance Staph. aureus.