Isolation and Identification of Porcine Circovirus 2 from Cases of Respiratory Disease and Postweaning Multisystemic Wasting Syndrome in Pigs

摘要

Porcine circovirus disease (PCVD), an emerging viral disease of economic importance, represents several disease manifestations principally caused by porcine circovirus 2 (PCV2). Prevalence of distinct PCV2 genotypes in pig population necessitates to study their replication potential in cell line in order to establish and compare infectivity and pathogenecity. Virus isolation forms an important aid in PCVD diagnosis, especially when multifactorial origin of disease condition is suspected. In the present study, isolation of PCV2 was accomplished by serially passaging the inoculum prepared from suspected tissue samples up to fourth passage in PK–15 cell line. Further, the confirmation of replication in cell line was done by immunoperoxidase monolayer assay and polymerase chain reaction. Information regarding virus isolation and identification of PCV2 isolates circulating in Indian pigs is scarce. In the present work, PCV2 was successfully isolated in PK–15 cell line and demonstrated their replication in cytoplasm and in nucleus by immunoperoxidase monolayer assay. Thus, virus isolation together with PCR assay helped to establish the PCV2 etiology in pneumonia and wasting of pigs encountered in the present study