Molecular Identification of Candida Species Isolated from Ears of Dogs Infected with Otitis externa by Detecting Internal Transcript Spacer (ITS1 and ITS4) in Sulaimania, Iraq

摘要

The goal of this study was to determine internal transcribed spacer/5.8S ribosomal DNA (rDNA) to detect Candida spp. in dogs with Otitis externa. PCR-based detection of internal transcribed spacer 2 (ITS2) regions of the rRNA genes was evaluated as a means of fungal identification by using Internal transcribed spacers 1 (ITS1) and Internal transcribed spacers 4 (ITS4). These were amplified by PCR to detect fungal DNA. Sixty swab ear samples from domestic dogs with and without O. externa were examined for clinical signs including head shaking, scratching the external ear flaps, bad odor, obstraction of the ear canal, anorexia, emaciation and auricular discharge (unilateral in 20 and bilateral in 10 dogs). The isolation of Candida species was performed from the external otitic canal in all animals. Yeast species isolated in 30 cases involving mainly Candida spp. (20 cases) and more often Aspergillus niger (5 cases), Penicellium spp. (3 cases) and Aspergillus fumigatus (2 cases). Twenty isolates of Candida used to test the selected primers and conditions of the PCR. Molecular fungal identification is successful in most isolates; with a band size 600-800 bp. This study concluded that size of PCR-amplified ITS2 region DNA is a rapid and reliable method to identify clinically significant yeasts.