Phylogenetic Analysis of Peste des Petits Ruminants Virus Isolated from District Gujranwala, Pakistan

摘要

Peste des petits ruminants (PPR) disease is an endemic disease in Pakistan and its neighboring countries. The blood samples from sheep and goats suffering from PPR were taken from district Gujranwala Pakistan to investigate the molecular nature and geographical distribution of Peste des petits ruminants virus (PPRV) circulating in Gujranwala. The nucleoprotein (N) was amplified by using RT-PCR, product was further subject to conventional PCR to get multiple copies of it, then sequenced and aligned with the available corresponding sequences and phylogenetic tree was constructed. The results provide strong evidence of presence of expected Asian lineage IV. The PPRV isolates perceived in district Gujranwala showed high degree of homology with the PPRV isolates of Tajikistan. The current study delivered information of topical spread of PPRV lineage IV from neighboring proximity to the small ruminant in district Gujranwala. Thus it is very crucial to characterize the PPRV in the whole country to control the spread of disease