CHARACTER OF CYTOCHROME OXIDASE 1 GENE (CO1) IN MITOCHONDRIAL DNA DAMSELFLY Agriocnemis femina FROM LINOW LAKE, TONDANO LAKE AND MOAT LAKE AT NORTH SULAWESI

摘要

This study aims to find the character gene cytochrome oxidase subunit 1 (CO1), mitochondrial DNA damselfly ( Agriocnemis femina ) derived from Tondano Lake, Linow Lake and Lake Moat. The research was conducted in several stages, sampling, extraction and purification of total DNA, CO1 gene amplification and sequencing. Sequencing results were analyzed using Geneous Software 5.6.4. Further alignment analysis using NCBI BLAST (www.ncbi.nih.gov). Although the sample morphology Damselfly used for DNA analysis showed differences in the characteristics of phenotypically plastic. CO1 gene sequencing results of the six samples show the sequence of nitrogenous bases Damsefly have identical percentage (similarity), 99 percent or it can be said based gene CO1, six samples are the same species. Similarity 6 Damsefly samples from three different ecosystems that Tondano Lake, Lake and Lake Moat Linow average above 99% with Agriocnemis femina. Some differences morphological characters of each sample from a different lake ecosystems is not sufficient to give effect to CO1 gene mutations in mitochondrial DNA or gene conservation CO1 at Demselfly that live in three lake ecosystems is very high.