Hepatoprotective Activity of Chitosan Nanocarriers Loaded with the Ethyl Acetate Extract of a Stenotrophomonas sp. Bacteria Associated with the Red Sea Sponge Amphimedon ochracea in CCl4 Induced Hepatotoxicty in Rats

摘要

The present study investigated the protective role of chitosannano-carriers loaded with the ethyl acetate extract of a Stenotrophomonas sp. bacteria associated with the Red Sea Sponge Amphimedon ochracea CCl4 induced hepatic damage in rats using the hepatological parameters. Healthy 80 male Sprague-Dawley rats weighed 50 - 80 gm were used. The animals were randomly divided into eight groups. Serum ALT and AST, albumin, direct buluibin and histopathological examinations were investigated. Liver targeting of the drugs has been achieved after administration of drug loaded spherical particles. This mode of administration of drugs enhanced its overall delivery to the liver via passive targeting. The present study revealed that the treatment with ethyl acetate bacterial extract (10 mg/Kg body weight) protected rats from hepatotoxic action of CCl4. Usage of chitosan nanoparticles loaded with the bacterial extract as a drug delivery system has greatly improved the curative effect of bacterial extract. Moreover, histopathological studies showed marked reduction in fatty degeneration and centrizonal necrosis in animals receiving the nano-chitosan loaded with the bacterial extract along with ethanol compared to the control group.