Catalase Enzyme Activity and Protein Estimation In Different Varieties if Sorghum (Sorghum bicolor)

摘要

Protein estimation and catalase (Sorghum bicolor) was carried out in four different varieties of sorghum. Seeds of varieties viz., DSV 4, DSV 5, CSV 216R and M35 washed with pinch of mercuric chloride and repeatedly washed with distilled water were sown in sterile soil in pots. Estimation of protein is done through Folin-Cio-Calteau method. DSV 4 showed the highest concentration at 660 nm and CSV 216R showed least concentration in standard BSA graph. The blue coloured bands were observed immediately against bright background. The blue coloured bands indicated the presence of catalase enzyme which protect cells from toxic by product produced in cells . catalase isoforms were prominent in shoot samples and faint bands were observed in root samples.