Research on Inhibitory Effect of Momordica charantia L. Polyphenol on Vascular Endothelial Intercellular Adhesion Molecular-1 (ICAM-1) mRNA Abnormal Expression

摘要

The study held a research on the anti-oxidative damage effect and mechanism of Momordica charantia L. Polyphenol from gene level. Method: it tested the influence of Momordica charantia L. Polyphenol to human umbilical veins blood vessel endothelial cell strain (CRL-1730) ICAM-1 mRNA cellular expression caused by oxidative damage with RT-PCR method. Result: the experimental result indicated that the luminance and area values of electrophoretic band of 4, 5, and 6 with different doses of Momordica charantia L. Polyphenol for protection were all lower than positive control group, while higher than normal control group, furthermore, it presented a state that be increasing with the augment of Momordica charantia L. Polyphenol dose. The degree of protection of the three dose groups, high, medium and low, were separately 91.1, 82.7 and 37.3%, respectively after making a comparison in each group. Conclusion: Momordica charantia L. Polyphenol can inhibit cell adhesion molecular-1 expression and make a protective effect to vascular endothelial cell damage.