THE INFLUENCE OF METHYL JASMONATE AND SALICYLIC ACID ON SECONDARY METABOLITE PRODUCTION IN REHMANNIA GLUTINOSA LIBOSCH. HAIRY ROOT CULTURE

摘要

Rehmannia glutinosa hairy roots were used to evaluate the effect of methyl jasmonate (MeJa) and salicylic acid(SA) on increase of root biomass and production of iridoids (catalpol, harpagide) and phenylethanoids (verbascosideand isoverbascoside). The elicitors were added to 23-day-old culture separately at concentrations between50 and 200 μM or in combinations at concentrations of 50 and 100 μM. Roots were harvested 72 h and 120 hafter elicitation. The type of elicitor, its concentration and exposure time were found to strongly affect the contentof each analyzed compound. A 72-hour treatment with 200 μM MeJa was the most effective in increase of verbascosidecontent (60.07 mg·DW-1 equivalent to 845.45 mg·L-1) and isoverbascoside (1.77 mg·DW-1 equivalent to24.94 mg·L-1): these respective amounts were roughly 10- and 6.4-fold higher than the control values (unelicitedroots). Exposure to 150 μM MeJa provided optimal harpagide content after 72 hours (0.136 mg·DW-1; 7.5-foldincrease compared to the control), and catalpol content after 120 hours (up to 2.145 mg·DW-1). The combinationof MeJa and SA also resulted in higher levels of secondary metabolites compared to the control culture, althoughthese levels were lower than those observed for MeJa alone at the optimal concentration and exposure time. SAalone was less efficient in enhancing metabolite production than MeJa.