Properties of polyphenol oxidase obtained from Musa acuminata????????????balbisiana Colla cv. ?￠???? Pisang Awak ?￠???? pulp and peel Propiedades de polifenoloxidasa obtenido de la pulpa y piel de Musa acuminata????????????balbisiana Colla cv. ?￠???? Pisang Awak ?￠????

摘要

The characteristics of crude polyphenol oxidase (PPO) extracted from the peel and pulp of the plantain Awak ( Musa acuminata ???????????? balbisiana ) were investigated to provide useful information on the plantain during processing operations. The optimum pH-activity of crude PPO for the peel and pulp is 5.5 and 6.5, respectively. Optimum temperature-activity for the peel and pulp was 30???????°C. The K m and V max of the crude PPO peel were 4.92????mM and 0.702 Abs/min, respectively, while the K m and V max for the crude PPO pulp were 3.77????mM and 0.513 Abs/min, respectively. Crude PPO from both peel and pulp exhibited similar trends in thermostability. Crude Awak PPO from peel and pulp was indicated to be 100% inhibited by 1????mM of l -cysteine, ascorbic acid, and sodium metabisulphite. Citric acid was found to be moderately effective for PPO inhibition, and NaCl was demonstrated to be a weak inhibitor. Las caracter???-sticas de las polifenoloxidasa (PFO) extra???-das de la piel y pulpa sin refinar del pl????tano Awak ( Musa acuminata ???????? balbisiana ) fueron investigadas para obtener informaci???3n ???otil sobre el pl????tano durante las operaciones de procesado. El pH ???3ptimo para PFO de la piel y pulpa es 5,5????y 6,5, respectivamente. La temperatura ???3ptima para la piel y pulpa fue 30???????°C. Las K m y V max de las PFO de piel sin refinar fueron 4,92????mM y 0,702 Abs/min, respectivamente, mientras las K m y V max de las PFO de pulpa sin refinar fueron 3,77????mM y 0,513 Abs/min, respectivamente. Las PFO de ambas piel y pulpa mostraron tendencias similares en termoestabilidad. Las PFO de piel y pulpa sin refinar de Awak resultaron ser 100% inhibidas por 1????mM de l -ciste???-na, ????cido asc???3rbico y metabisulfito de sodio. El ????cido c???-trico se mostr???3 moderadamente efectivo para inhibici???3n de PFO y se demostr???3 que NaCl es un inhibidor d????bil.