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Meiotic behavior of lulo species, Solanum sp

机译:茄属物种夜蛾物种的减数分裂行为

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An analysis of meiotic behavior of lulo species S. hirtum, S. quitoense and S. sessiliflorum, following the conventional methodology for studies of microsporogenesis was realized. Flower buds were taken at different stages of development, fixing them for 24 hours in a solution of three parts of ethanol per one part of acetic acid, saturated with traces of ferric chloride crystals. For the preparation of the slides following the technique of squash, releasing pollen mother cells and finally made the observations under light microscopy. The analysis showed that meiosis occurs in anther ranging from 2.79 to 4.45 mm. Meiotic normality was 100% for both meiosis I and II. The meiotic index in all three species was 99,98% indicating that they are good parental for use in crossbreeding programs. The three species have the same number of chromosomes (2n = 2x = 24). The frequency of abnormalities during the meiotic process was lower for S. hirtum, and high for S. quitoense, however, the pollen viability was of great magnitude (91.2-97.3%).
机译:按照常规的微孢子发生研究方法,实现了对流线型S. hirtum,S。quitoense和S. sessiliflorum减数分裂行为的分析。花蕾在发育的不同阶段拍摄,将它们在三份乙醇/一份乙酸中的溶液中固定24小时,每份乙酸中充满了微量的氯化铁晶体。为了按照壁球技术制备载玻片,释放出花粉母细胞,最后在光学显微镜下观察。分析表明减数分裂发生在2.79至4.45mm的花药中。减数分裂I和II的减数分裂正常率均为100%。这三个物种的减数分裂指数均为99.98%,表明它们是育种计划中的优良亲本。这三个物种具有相同数量的染色体(2n = 2x = 24)。减数分裂过程中异常的发生频率,对于水生葡萄球菌而言较低,而对于水产链球菌则较高,但是,花粉生存力的幅度很大(91.2-97.3%)。

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