Clonal multiplication in vivo and in vitro of the native forest species Aniba perutilis Hemsl

摘要

A methodology for the in vitro and in vivo establishment of Aniba perutilis, a native colombian tree which is currently critically endangered, was performed. In the first stage seeds were treated using NaOCl, HgCl2, and AgNO3 to achieve aseptic germination. In order to induce shoot formation, WPM medium was used and phytohormones BAP, KIN, IAA, NAA and GA3 were evaluated at different concentrations. In the rooting stage NAA and IBA were used in different concentrations and culture media, as well as half-strength MS and WPM mediums combined with different concentrations of IAA and NAA. For in vivo multiplication stage, juvenile plants previously established in nursery were used to evaluate three types of cuts defined by the distance between the base of the plant and where the cut was done: distal (11-14 cm), middle (7-8 cm) and basal (3-4 cm), in order to obtain ‘donor plants’ able to donate axillary buds and mini-cuttings for future rooting. The use of NaOCl for 15 minutes consolidates as optimal disinfection treatment, yielding a survival rate greater than 60%. The WPM medium supplemented with BAP (3 mg/L) and GA3 (1.5 mg/L) allowed the production of 0.6 shoots/explant with an average length of 0.82 cm. However, the low rooting capacity of the explants suggests that this species could be labeled as recalcitrant to in vitro culture. In vivo conditions, cuts made at the middle height of the plants promoted the formation of 1.33 shoots/explant and 75% rooting in mini-cuttings, becoming these results in a