Evaluation of Genetic Damage in Oreochromis mossambicus Exposed to Selected Nanoparticles by Using Micronucleus and Comet Bioassays

摘要

The purpose of the present study is to extend knowledge on the adverse effects of nanoparticles by evaluating genotoxicity as environmental risk assessment in Oreochromis mossambicus. Fish were exposed to sublethal concentrations of the selected nanoparticles, namely silicon dioxide (SiOsub2/subNPs-12mg/L), aluminium oxide (Alsub2/subOsub3/subNPs-4mg/L), titanium dioxide (TiOsub2/subNPs-16.4mg/L) and iron oxide (Fesub3/subOsub4/subNPs-15mg/L) for short-term (24, 72 and 96 h) and long-term durations (15, 30 and 60 days). Genetic damages such as cytoplasmic, nuclear and DNA damage were measured in the erythrocytes of fish by using standard genotoxicity tests such as micronucleus test and comet assay. The frequencies of micronuclei along with nuclear and cytoplasmic abnormalities were scored and compared with the control group. The intensity of micronuclei along with other nuclear and cytoplasmic anomalies are found to be increased significantly (pOreochromis mossambicus.