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Significant modulation of the hepatic proteome induced by exposure to low temperature in Xenopus laevis

机译:非洲爪蟾暴露于低温引起的肝蛋白质组的显着调节

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The African clawed frog, Xenopus laevis , is an ectothermic vertebrate that can survive at low environmental temperatures. To gain insight into the molecular events induced by low body temperature, liver proteins were evaluated at the standard laboratory rearing temperature (22°C, control) and a low environmental temperature (5°C, cold exposure). Using nano-flow liquid chromatography coupled with tandem mass spectrometry, we identified 58 proteins that differed in abundance. A subsequent Gene Ontology analysis revealed that the tyrosine and phenylalanine catabolic processes were modulated by cold exposure, which resulted in decreases in hepatic tyrosine and phenylalanine, respectively. Similarly, levels of pyruvate kinase and enolase, which are involved in glycolysis and glycogen synthesis, were also decreased, whereas levels of glycogen phosphorylase, which participates in glycogenolysis, were increased. Therefore, we measured metabolites in the respective pathways and found that levels of hepatic glycogen and glucose were decreased. Although the liver was under oxidative stress because of iron accumulation caused by hepatic erythrocyte destruction, the hepatic NADPH/NADP ratio was not changed. Thus, glycogen is probably utilized mainly for NADPH supply rather than for energy or glucose production. In conclusion, X. laevis responds to low body temperature by modulating its hepatic proteome, which results in altered carbohydrate metabolism.
机译:非洲爪蛙非洲爪蟾(Xenopus laevis)是一种可以在低环境温度下生存的外热脊椎动物。为了深入了解低体温引起的分子事件,在标准实验室饲养温度(22°C,对照)和低环境温度(5°C,冷暴露)下评估了肝蛋白。使用纳流液相色谱与串联质谱联用,我们鉴定了58种丰度不同的蛋白质。随后的基因本体分析表明,酪氨酸和苯丙氨酸的分解代谢过程受到冷暴露的调节,这分别导致肝酪氨酸和苯丙氨酸的减少。类似地,参与糖酵解和糖原合成的丙酮酸激酶和烯醇酶的水平也降低了,而参与糖原分解的糖原磷酸化酶的水平增加了。因此,我们测量了各个途径中的代谢物,发现肝糖原和葡萄糖水平降低了。尽管由于肝红细胞破坏引起的铁蓄积使肝脏处于氧化应激状态,但肝NADPH / NADP的比例没有改变。因此,糖原可能主要用于供应NADPH,而不是用于产生能量或葡萄糖。总之,X。laevis通过调节其肝脏蛋白质组对低体温做出反应,这导致碳水化合物代谢的改变。

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