Evaluation of a Spontaneous Canine Model of Immunoglobulin E-Mediated Food Hypersensitivity: Dynamic Changes in Serum and Fecal Allergen-Specific Immunoglobulin E Values Relative to Dietary Change

摘要

Thepurposeofthepilotstudyreportedherewastoevaluateserumandfecaltotalandallergen-specificimmunoglobulinE(IgE)responsestodietarychangeinfiveMaltesexbeagledogswithsuspectedfoodhypersensitivity,comparedwiththoseoffiveclinicallynormaldogs.Clinicalparameters(pruritus,otitis,anddiarrhea)improvedintheMaltesexbeagledogsduringfeedingofanoveldiet,andsignswereexacerbatedbyoralallergenprovocation.Relativeconcentrationsofserumandfecalwheat-,corn-,andmilk-specificIgEweredeterminedbyuseofanELISA.Theonsetofclinicalsignsofdiseasewasaccompaniedbyanincreaseinserumallergen-specificIgEconcentrations.Incontrast,changesinclinicalsignsofdiseaseorallergen-specificIgEvalueswerenotseeninthecontrolgroupundergoingthesameregimen.TotalserumIgEconcentrationwasmeasuredbyuseoftheELISA,andcomparisonwithknownquantitiesofamonoclonalIgEallowedabsolutevaluestobereported.ValueswerehighintheMaltesexbeaglecolony(7to34#59157;g/ml),comparedwiththoseinthecontroldogs(0.7to6#59157;g/ml).TotalserumandtotalfecalIgEconcentrationsdidnotchangeineithergroupduringthestudy.Althoughallergen-specificIgEwasdetectedinthefecesofbothgroups,significantinterassayvariabilitymadeinterpretationoftheresultsdifficult.TheauthorsconcludedthattheseMaltesexbeagledogssatisfiedthecurrentlyrecognizedclinicalcriteriaforthediagnosisofcaninefoodhypersensitivity.Furthermore,theclinicalandserologicresponsesseeninthesedogsinresponsetooralallergenprovocationsuggestthatthismaybeausefulmodelforthestudyofspontaneousfoodhypersensitivity.