Swine Genome Sequencing Consortium (SGSC): A Strategic Roadmap for Sequencing The Pig Genome

摘要

The Swine Genome Sequencing Consortium (SGSC) was formed in September2003 by academic, government and industry representatives to provide internationalcoordination for sequencing the pig genome. The SGSC’s mission is to advancebiomedical research for animal production and health by the development of DNAbasedtools and products resulting from the sequencing of the swine genome. Duringthe past 2 years, the SGSC has met bi-annually to develop a strategic roadmap forcreating the required scientific resources, to integrate existing physical maps, and tocreate a sequencing strategy that captured international participation and a broadfunding base. During the past year, SGSC members have integrated their respectivephysical mapping data with the goal of creating a minimal tiling path (MTP)that will be used as the sequencing template. During the recent Plant and AnimalGenome meeting (January 16, 2005 San Diego, CA), presentations demonstrated thata human–pig comparative map has been completed, BAC fingerprint contigs (FPC)for each of the autosomes and X chromosome have been constructed and that BACend-sequencing has permitted, through BLAST analysis and RH-mapping, anchoringof the contigs. Thus, significant progress has been made towards the creation of aMTP. In addition, whole-genome (WG) shotgun libraries have been constructed andare currently being sequenced in various laboratories around the globe. Thus, ahybrid sequencing approach in which 3x coverage of BACs comprising the MTPand 3x of the WG-shotgun libraries will be used to develop a draft 6x coverage ofthe pig genome.