The tuberculin skin test: How safe is safe? - the tuberculins contain unknown forms capable ofreverting to cell-wall-deficient mycobacteria

摘要

Tuberculin is made from proteins derived from tubercle bacilli that have been “killed” byheating. Yet in both Zwadyk’s 1994 study and Bemer-Melchior’s 1999 investigations ‘heatkilled’tuberculosis and its related mycobacteria, whether in tuberculin, vaccination or otherwise,have dormant, practically indestructible cell-wall-deficient forms which can revert backto virulent TB bacilli “killed” ― by neither heat nor sterilization. The ability and actual preferenceof mycobacteria such as Mycobacterium tuberculosis and Mycobacterium bovis toform filterable, multi-shaped cell-wall-deficient (CWD) forms and spores in order to surviveunfavorable conditions has in fact been known for some time. But the possibility of PPD tuberculinsfor human use containing such potentially virulent CWD forms, even after autoclaving,sterilizing and ultrafiltration, has not. Autoclaved ultra-filtrates of the various mycobacteriaused to produce tuberculin skin tests, consisting of M. Tuberculosis, M. bovis, and M. aviumwere investigated. All samples were mixed with growth stimulant, incubated, and placedon a special nutrient medium with a 1% agar base. Within 2-10 days after incubation coloniesof a variety of non-acid-fast forms were noted, yet all of these proved, through PCR real timewith FAM probe to still have antigens in common with their classic tubercular parent-form,from which they originated. Moreover, in true cell-wall-deficient fashion, the isolates, uponguinea pig inoculation, did not immediately produce visible lesions, but nevertheless persisted.However, tissue homogenates of the infected animals, once placed on a growth-enhancingmedium showed cell-wall-deficient mycobacterial forms interspersed with classical acid-fastrods. And a repeated passage of such tissue homogenates back into non-infected guinea pigs,not only induced small mycobacterial granulomas in their livers, but a distinct increase in acid-fast rods. Moreover, similar cell-wall-deficient mycobacterial forms with acid-fast rods occurredwhen embryonated chicken eggs were inoculated with PPD tuberculins as well.The autoclaved and supposedly “sterilized” purified protein derivative [PPD] used in tuberculinskin tests contain cell-wall-deficient forms capable of eventually reverting back to virulentacid-fast tuberculosis, both typical and atypical.