Genome wide gene expression analysis of nasal mucosa from patients with chronic rhinosinusitis and nasal polyposis stimulated with staphylococcal enterotoxin B

摘要

IntroductionColonization of the nasal mucosal with Staphylococcusaureus and the production of superantigenic enterotoxinsby the bacteria are crucial amplifying factors of thepro-inflammatory mechanisms operating in chronicupper airways diseases such as chronic rhinosinusitisand nasal polyposis.MethodsNasal polyp tissue from 10 patients with chronic rhinosinusitisasal polyposis and inferior turbinate from8 healthy subjects were obtained fragmented and culturedin presence and absence of S. aureus enterotoxin B(0.5μg/ml) for 24 hours. Then total RNA was isolated,transcribed to cDNA and used for microarray analysisusing the Affymetrix Human Gene 2.1 ST Array. Data wasthen corrected and differentially expressed genes wereselected according to the corrected P value < 0.05 and achange fold higher than 2. Pathway overrepresentationanalysis was then performed in each group of genes.ResultsThe number of genes showing differential expressionbetween nasal polyp and control tissues as well as thegenes and pathways differentially regulated after stimulationwith S. aureus enterotoxin B in each of the groupsis described in Table 1.ConclusionsWe demonstrated that S. aureus enterotoxin B mayinfluence important pathways linked to T-cell receptor,interferon signalling and adaptative immune response.