Ahmed Gafar, Mohammed Bashandy, Sayed Bakry, Mahmoud A. Khalifa, and Walid Abu Shair

摘要

Lithocholic acid (LCA) is toxic to human prostate cancer cells. We previously studied the effects of LCA on cell viability, cell proliferation and mitochondrial function in LNCaP and PC-3 prostate cancer cell lines. In the present study, we investigated the induction of extrinsic apoptosis by LCA in androgen independent PC-3 and DU-145 cells. In PC-3 and DU-145 cells, LCA triggered extrinsic apoptosis pathways and induced typical extrinsic apoptosis -related proteins, such TRIAL, FasL, FADD, DR5, DR4 and Cleaved caspase8. Treatment of prostate cancer cells with IETD AFC (10 μM), an inhibitor of Caspase 8 partially inhibited apoptosis that induced by LCA. Also, we found Death receptor (DR5) silencing partially Blocked LCA to induced cell death. Collectively, these results indicate that extrinsic apoptosis induced by LCA in PC-3 and DU-145 play a secondary role in cell death and there might be another cell surface receptor that LCA triggers to induce cell death.