Polyethylene glycol increases the penetration of bovine viral diarrhea virus, vesicular stomatitis virus and bovine respiratory syncytial virus in cultured cells

摘要

> face="Verdana, Arial, Helvetica, sans-serif" size="2">The low efficiency of penetration of some viruses in cultured cells may represent an obstacle for viral isolation and/or viral multiplication in tissue culture. This study investigated the effect of polyethylene glycol (PEG) on the penetration and replication of seven bovine enveloped viruses in culture cells. Penetration efficiency was measured by counting the number of viral plaques produced in bovine kidney cells (MDBK). The addition of 5% PEG (molecular weight 6.000) to the viral inoculum containing 100 TCID₅₀ mL-1 (tissue culture median infectious dosis) of each virus, during adsorption for 2h at 37°C, resulted in a significant increase in the number of plaques for bovine viral diarrhea virus (BVDV) (increase of 3.4-fold), vesicular stomatitis virus (VSV) (2.2-fold) and bovine respiratory syncytial virus (BRSV) (1.5-fold). The addition of 5% PEG to the inoculum of bovine herpesviruses 1, 2 and 5 (BoHV-1, BoHV-2 and BoHV-5) did not increase the number of viral plaques. On the other hand, PEG produced a reduction in the number of plaques by bovine parainfluenza virus (bPI-3V) (1.4-fold). Furthermore, the addition of 5% PEG produced a 10- to 1000-fold increase in the sensitivity of BVDV detection in the serum of three persistently infected calves; and doubled the sensitivity of detection of BRSV in nasal secretions of two experimentally infected sheep. These results demonstrate that PEG enhances the efficiency of infection by BVDV, VSV and BRSV in cultured bovine cells and therefore may be used to increase the sensitivity of virus detection in clinical samples (viral isolation), and/or to increase virus titers in cell cultures.