Characterization of chromosome complement in Filifolium sibilicum (L.) Kitamura by aceto-orcein, CMA, DAPI and FISH 5S and 45S rDNA

摘要

The chromosome characteristics of Filifolium sibilicum (2n=18) were studied regarding the karyotype by aceto-orcein, locations of the G-C and A-T rich regions by CMA and DAPI, and FISH. The karyotypes of six representative individuals in three localities such as two sites in Inner Mongolia Autonomous Region, The People's Republic of China and one site in Buryat Republic in Russian Federation consisted commonly of 12 median-centromeric, four satellited median-centromeric and four submedian-centromeric chromosomes. CMA-stained the whole chromosomes, but four brighter bands were on terminal positions of four chromosomes and were convenient with the four sat chromosomes. DAPI-stained the whole metaphase chromosomes. Applying FISH using the biotin labeled probe of 5S rDNA and digoxigenin labeled pTa71 probe of 45S rDNA on the mitotic chromosomes showed four yellow colored signals of the 5S rDNA and four red signals that were terminally co-localized, which were obtained after hybridization of the probes with two submedian and two subterminal metaphase chromosomes.