The histochemical effects of Immunosuppressive Drug Azathioprine on the Cytoarchitecture of the Testes of Adult Wistar Rats (Rattus norvegicus)

摘要

In this work, we studied the histochemical effects of ascorbic acid on immunosuppressive drug-azathioprine-induced alterations in the testis of adult Wistar rats. The rats (n=30) with mean weight 210±2.65 g were assigned into four treatment (n=24) and control (n=6) groups. The rats in the treated groups II and IV received 10 and 20 mg/kg/Bwt of AZA respectively, rats in the treated groups III and V received 10 and 20 mg/kg/Bwt of AZA combined with 25 mg/kg/Bwt and 50 mg/kg/Bwt of ascorbic acid respectively, while rats in control group received equal ml of distilled water for 21 days after which they were sacrificed; testes were excised, weighed and fixed in Bouins’ fluid for histochemical studies of reticulin and collagen. The histochemical findings of testis sections indicated cyto-architectural distortions in the treated groups as compared with the control group. The findings show that ascorbic acid cannot ameliorate the damages caused by azathioprine.