SYNTHESIS, SPECTROSCOPIC INVESTIGATION, AND MOLECULAR INTERACTIONS BETWEEN ACIVICIN AND CdSe QUANTUM DOTS

摘要

Water-Soluble and fluorescent thiol capped CdSe quantum dots (QDs) were synthesized by chemical rout. The structure of CdSe QDs was investigated by means of X– ray diffraction (XRD), energy–dispersive X–ray analysis (EDX), UV– visible, Fourier transform infrared (FT–IR) spectroscopy and transmission electron microscop y (TEM). The crystal phase of the CdSe QDs confirmed with XRD diffraction technique. The average size of CdSe QDs was calculated to be 3 nm b y different spectroscopic techniques. CdSe capped with mercaptopropionic acid (MPA) shows a characteristic fluorescent peak at 590 nm, which is highly dependant on the pH value. The interaction of the water-soluble MPA– CdSe and acivicin (AC) was investigated in 50 mM sodium acetate buffer pH 7.4 by fluorescence spectroscopy. The interaction between acivicin and MPA–CdSe shows a dynamic quenching process. Analysis of the circular dichorism (CD) spectra indicated higher interaction of the MPA–CdSe-AC complex with human serum albumin than the individual AC drug.