Comparison of Culture, Real-Time DNA Amplification Assay and Ehrlich-Ziehl-Neelsen for Detection of Mycobacterium tuberculosis

摘要

Objective: Mycobacterium tuberculosis is still a substantial health problem universally. Although culture is the gold standard method, reliable, rapid and new methods are required for effective struggle with disease. We retrospectively compared the results of Ehrlich-Ziehl-Neelsen (EZN) stain and real-time DNA amplification assay (BD ProbeTec ET system) with culture. Study Design: Retrospective study. Material and Methods: A total of 703 samples, 182 pulmonary and 521 extra pulmonary, collected from 630 patients between May 2008 and February 2011 were evaluated. Culture was considered the gold standard. Results: For pulmonary specimens, sensitivity, specificity, positive predictive and negative predictive values of BD ProbeTec ET and EZN were calculated to be 100%, 98.8%, 87.5%, 100% and 71.4%, 98.8%, 83.3%, 97.6%, respectively. For extra pulmonary specimens, sensitivity, specificity, positive predictive and negative predictive values of BD ProbeTec ET and EZN were calculated to be 80%, 98.7%, 76.9%, 98.9% and 24%, 98.3%, 42.8%, 96.2%, respectively. Conclusion: According to these results, we suggest that the BD ProbeTec ET system is more reliable than EZN. In addition, the BD ProbeTec ET system produces faster results. Based upon these results, we consider that the BD ProbeTec ET system may be employed in the diagnosis of M. tuberculosis. Turkish Ba?l?k: Mycobacterium tuberculosis'in tespitinde Kültür, BD ProbeTec ET ve EZN'nin kar??la?t?r?lmas? Anahtar Kelimeler: Mycobacterium tuberculosis, BD ProbeTec ET, kültür Ama?: Mycobacterium tuberculosis hala evrensel bir sa?l?k problemi olarak varl???n? devam ettirmektedir. Kültür alt?n standart y?ntem olmas?na ra?men, hastal?kla etkin mücadelede güvenilir ve h?zl? yeni metodlara ihtiya? vard?r. Biz, bu ama?la retrospektif olarak Ehrlich-Ziehl-Neelsen (EZN) boyama ve BD ProbeTec ET sistem sonu?lar?n? kültür ile kar??la?t?rd?k. Gere? ve Y?ntemler: May?s 2008 ve ?ubat 2011 tarihleri aras?nda 630 hastadan toplanan, 182'si pulmoner ve 521'i ekstra pulmoner, toplam 703 ?rnek de?erlendirildi. Kültür alt?n standart olarak kabul edildi. Bulgular: Pulmoner ?rnekler i?in duyarl?l?k, ?zgüllük, pozitif prediktif ve negatif prediktif de?erler BD ProbeTec ET ve EZN i?in s?ras?yla %100, %98.8, %87.5, %100 ve %71.4, %98.8, %83.3, %97.6 olarak hesapland?. Ekstra pulmoner ?rnekler i?in duyarl?l?k, ?zgüllük, pozitif prediktif ve negatif prediktif de?erler BD ProbeTec ET ve EZN i?in s?ras?yla %80, %98.7, %76.9, %98.9 ve %24, %98.3, %42.8, %96.2 olarak hesapland?. Sonu?: Bu sonu?lara g?re, BD ProbeTec ET sisteminin EZN'den daha güvenilir oldu?u g?rülmü?tür. Ek olarak, BD ProbeTec ET sistemi h?zl? sonu? vermektedir. Bu verilere dayanarak, BD ProbeTec ET sisteminin M. tuberculosis tan?s?nda h?zl? ve güvenilir bir y?ntem olarak kullan?labilece?i dü?üncesindeyiz.