Overexpression of A613T and G462T variants of DNA polymerase β weakens chemotherapy sensitivity in esophageal cancer cell lines

摘要

Background Human DNA polymerase β (polβ) is a small?monomeric?protein that is essential for short-patch base excision repair. It plays an important role in regulating the sensitivity of tumor cells to chemotherapy. Methods We evaluated the mutation of?polβ?in a larger cohort of esophageal cancer (EC) patients by RT-PCR and sequencing analysis. The function of the mutation was evaluated by?CCK-8,?in vivo tumor growth, and flow?cytometry?assays. Results There are 229 patients with the?polβ?mutation, 18 patients with A613T mutation, 12 patients with G462T mutation among 538?ECs.?Analysis results of survival time showed that EC patients with A613T, G462T mutation had a shorter survival than the others ( P ?/??groups ( P Conclusions In this study, we discovered A to T point mutation at nucleotide 613 (A613T) and G to T point mutation at nucleotide 462 (G462T) in the?polβ?gene through?538 EC patients cohort study. A613T and G462T variant of DNA polymerase β?weaken?chemotherapy?sensitivity?of esophageal cancer.