In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii

摘要

Objective: Ivermectin and sulphadiazine were tested individually to determine their in vitro effects on Toxoplasma gondii grown in human epidermoid larynx carcinoma (Hep-2) cell culture. Study Design: In-vitro study. Material and Methods: Toxoplasma growth was quantities by an enzyme immunoassay performed directly on the fixed cultures, using a rabbit anti-T. gondii immunoglobulin G as the first antibody and a phosphatase-labeled anti-rabbit immunoglobulin G as the second antibody. For each drug, regression models were used to quantify the relationship between optical density values and antimicrobial agent concentrations in the cultures. Results: The 50% inhibitory concentrations (IC50) of ivermectin and sulphadiazine were found to be 0.2 μg/mL and 7.3 μg/mL after 48 h of exposure, respectively. None of the concentrations tested for each drugs demonstrated toxicity to Hep-2 cells after 72 h of incubation. Conclusion: These results indicate that ivermectin significantly inhibited replication of the tachyzoites of T. gondii RH strain. Turkish Ba?l?k: ?vermektin ve sulfadiazinin toxoplasma gond??'ye etkinliklerinin in vitro de?erlendirilmesi Anahtar Kelimeler: Toxoplasma gondii, ivermektin, sulfadiazin, in vitro, Hep-2 Ama?: ?vermektin ile sulfadiazinin, in vitro T. gondii ile enfekte insan larenks epidermoid karsinom (Hep-2) hücrelerinde anti-toxoplasma aktiviteleri ara?t?r?lm??t?r. Gere? ve Y?ntemler: Antimikrobiyal ajanlar?n anti-Toxoplasma aktiviteleri hücre kültür tabanl? ELISA ile de?erlendirilmi?tir. Primer antikor olarak tav?an anti-T gondii immunglobulin G sekonder antikor olarak alkalen fosfataz i?aretli anti-rabbit immunglobulin G kullan?lm??t?r. Her ila? i?in, optik dansite de?erleri ile antimikrobiyal ajan konsantrasyonlar? aras?ndaki ili?ki regresyon analizi uygulanarak de?erlendirilmi?tir. Bulgular: ?vermektin ve sulfadiazinin inkübasyonun 48. saatinde, %50 inhibit?r konsantrasyon (IC50) de?erleri s?ras?yla 0.2 μg/ml ve 7.3 μg/ml olarak hesaplanm??t?r. Ayr?ca her iki ilac?n test edilen konsantrasyonlar?n?n Hep-2 hücrelerine toksik olmad??? saptanm??t?r. Sonu?: Bu sonu?lar; ivermektinin in vitro T.gondii RH su?u takizoit replikasyonunu anlaml? olarak inhibe etti?ini g?stermi?tir.