RESEARCHES CONCERNING THE QUALITY OF THAWED STALLION SEMEN

摘要

During the 2007th breeding season, ten frozen semen samples (1-6 from a stallion from SD Jucu stable and 7-10 from the Centre Haras Nationaux, France) were took in study. The semen was collected using phantom mare and CSU type artifficial vagina. Fresh semen was evaluated by macro and microscopic techics, and the criopreservation was made in the Cluj-Napoca Laboratory of Andrology and Reproduction Biotechnologies. It was performed fast thawing, appreciating the mobility, viability, with/without DIEE Ancu Dinca devices, concentration and morphological examination. The sperm post thawing motility was between 15% and 65% and the viability varied between 22% and 85%, being superior to the motility value. The DIEE device utilization had not a visible influence on post thawing sperm motility and viability. The concentration values, were between 53 mil/ml. and 105 mil/ml .The highest percentage of normal sperm, determined by Spermarc staining, was recorded in the case samples 7 and 8, with 90%, respectively 86%. The samples from Les Breviaires France sperm stocking centre, had a higher motility and viability percentage (65% motility and 85% viability), compare with the ones processed in our laboratory (55% motility and 75% viability). From the 6 ejaculates frozen at USAMV Cluj, 3 samples were considered to be good for insemination