APPLICATION OF REAL-TIME PCR FOR DETECTION OF ASPERGILLUS SPECIES IN ABORTED RUMINANT FOETUSES

摘要

This research has performed for detection of Aspergillus species (A. fumigatus, A. flavus, A. niger and A. terreus) in aborted bovine, ovine, caprine and camel foetuses by real-time PCR in Iran. After modification of real-time PCR on abomasal contents, from the total number of 970 samples, 141 (14.53%) gave positive results for Aspergillus species. Of them, 62 (17.71%), 33 (14.04%), 27 (12.05%) and 19 (11.8%) positive specimens were detected in bovine, ovine, caprine and camel foetuses respectively. Statistical analysis showed significant differences (P<0.05) between bovine and camel and bovine and caprine aborted foetuses. Aspergillus abortion was the most prevalent in cattle whereas camels tended to be the most resistant. This study was the first report of direct identification of Aspergillus species by real-time PCR in aborted bovine, ovine, caprine foetuses in Iran and camel foetuses in the world.