Moleculer cloning of vitelogenin gene in the Hard lipped barb (Osteochillus hasseltii C.V) and the effect of photoperiods on gene expression

摘要

Fish reproduction is influenced by photoperiods through regulation on endocrine gland activities in producing hormones necessary for gonadal growth and development, gametogenesis and reproductive cycles.? Research needs to be conducted to find out the effect of photoperiod on hard lipped barb reproductive performance by manipulating photoperiod. Design experimental with three photoperiod treatments, namely 14L:10D (control), 8L: 16D (short photoperiods) and 18L:6D (long photoperiods). Four aquaria consisting of nine fishes each were served as replicates.? Fishes were kept under these photoperiods for 8 weeks. The observed variable was the liver activities. Liver activity was evaluated by measuring gene expression of Vitelogenin. The normalized data were subjected to ANOVA followed by Tukey’s multiple-comparison tests. The length of cDNA Vitelogenin was 1136 bp. The vitelogenin precursors encoded cDNA consisted of 378 amino acids. The average of vitelogenin gene in each experimental group significantly increased according to longer photoperiods (P0.05) These results indicated that photoperiods had a stimulatory effect in improving hard lipped barb reproductive performance.