Callus Induction and Plant Regeneration in Eleven Perennial Ryegrass Cultivars

摘要

Perennial ryegrass (Lolium perenne L.) is an important grass species as turf and forage. The optimal combination of 2,4-D and BAP in the medium for callus induction and the optimal kinetin concentration in the medium for plant regeneration were investigated. Cultivar variation in the callus induction and plant regeneration of eleven perennial ryegrass was studied using the optimized culture medium. The results showed that the highest callus induction rate with the best callus quality assurance was obtained on MS medium containing 5 mg l ?￠????1 2,4-D and 0.05 mg l ?￠????1 BAP. MS medium containing 3 mg l ?￠????1 BAP, 1 mg l ?￠????1 NAA and 1 mg l ?￠????1 kinetin was most suitable for plant regeneration. Callus induction and plant regeneration of the eleven cultivars of perennial ryegrass were varied greatly, and higher overall plant regeneration rates were observed in cultivars Barlennium, Pace, Emerald and Premier.