SPR Based Fiber Optic Sensor for the Detection of Vitellogenin: An Endocrine Disruption Biomarker in Aquatic Environments

摘要

We have fabricated a fiber optic SPR biosensor for the detection of vitellogenin (Vg), an endocrine disruption biomarker in aquatic environments. The sensor was fabricated by immobilizing anti-Vg antibodies on the sensor surface. Control experiments performed on another similar protein, fetuin and on a sensing probe without the anti-Vg antibody receptor confirmed the specificity of the sensor. The limit of detection of the sensor was found to be as small as 1 ng/ml in our experimental window. The sensitivity of the sensor was 0.48 nm/(ng/ml). This sensor can be utilized for remote monitoring of the water bodies for any endocrine disruption phenomena.