Correlation of Matrix Metalloproteinase-9 (MMP-9) expression and bone destruction in Chronic Suppurative Otitis Media (CSOM) patients with cholesteatoma at Adam Malik General Hospital Medan-Indonesia

摘要

Introduction: Cholesteatoma is a cyst which coated by stratified squamous epithelium containing keratin epithelial desquamation. The recurrence and bone destruction arerelevant features in cholesteatoma pathophysiology, which makes cholesteatoma tend to be dangerous and difficult to treat. Recent studies have shown that variations in cellular systems of matrix metalloproteinase-9 (MMP-9) production contribute to the pathophysiology of cholesteatoma, especially in of bone erosion. Objective: The aim of this study is to determine the correlation between MMP-9 with bone destruction in CSOM with cholesteatoma patients. Materials and Methods: This study was conducted by using cross-sectional design to the 40 samples of cholesteatomas patients at Otorhinolaryngology Department of Adam Malik General Hospital in May 2016. The level of MMP-9 expression was assessed by immunohistochemical staining of cholesteatoma tissue. The immunoreactivity score was obtained by calculating the broad scores with the intensity score in the assessment. Then, the correlation test was also carried out between MMP-9 expression and bone destruction statistically. Results: This study found that most of participants were male gender patients (23 patients; 67.5%), 6-24 years old interval (23 patients; 67.5%), having intratemporal complication (35 patients; 87.5%); moderate level bone destruction (19 patients; 47.5%), and MMP-9 overexpression (34 patients; 85%). In addition, there was a significant correlation between MMP-9 expression and bone destruction (P = 0.000) in this study. Conclusion: There was a significant correlation between MMP-9 expression with bone destruction in this study.