Liquid formulation of endophytic Bacillus and its standardization for the management of Fusarium wilt in tomato

摘要

Liquid formulation of Bacillus subtilis strain EPCO16 was developed to enhance the shelf life and efficacy of the biocontrol agent besides easing the delivery of bioinoculants through microirrigation techniques. Super optimal broth with catabolic repressor (SOC) was found to be effective in promoting the population of B. subtilis EPCO16. The greater population level of B. subtilis EPCO16 was maintained in SOC amended with glycerol even after 12 months of storage without loss in cell feasibility, antagonistic activity (HCN and siderophore production) and plant growth promotion. The greater efficacy of liquid formulation of B. subtilis EPCO16 against Fusarium wilt enhanced the fruit yield compared to talc based formulation. The current study offered the successful technology for the development of liquid based formulation for bacterial biocontrol agent.