Analysis of genetic purity of rice hybrids and their parental lines using microsatellite markers

摘要

The present experiment was conducted with a view to identifying distinct microsatellite markers to assess fingerprinting of rice (Oryza sativa L.) hybrids, assessing variation within parental lines and testing the genetic purity of hybrid seed. Seventeen microsatellite markers were used for fingerprinting 15 rice hybrids and their parental lines. A total of 272 alleles were obtained from 17 microsatellite primer pairs with an average of 16 alleles per primer pair. The number of alleles amplified from each primer pair ranged from 10 to 23. Out of 17, seven microsatellite markers together differentiated all the 15 hybrids and the parental lines including two inbred variety. The microsatellite marker, RM72 distinguished parental lines of HB09 and Heera2, RM584 for Shakti and LP70, RM248 for BRRI hybrid dhan1, LP106, inbreds BRRI dhan28 and BRRI dhan29, RM211 for BRRI hybrid dhan2, ACI93024, RM219 for BRRI hybrid dhan3, ACI1 and LP108, RM20 for Gold and Tia and RM18 for SL08 to differentiate parental lines with its respective hybrids. RM229, the microsatellite marker distinguished CMS lines and their corresponding restorer lines for most the hybrids.