Simultaneous removal of nitrate and aniline from groundwater by cooperating heterotrophic denitrification with anaerobic ammonium oxidation

摘要

To investigate the performance of cooperating heterotrophic denitrification (HD) with anaerobic ammonium oxidation (ANAMMOX) in removing nitrate and aniline simultaneously from organic limitation combined contamination groundwater, batch cultures, and flow column experiments were constructed. The two experimental results showed that it was feasible to remove nitrogen and carbon simultaneously by indigenous HD and ANAMMOX bacteria existing ubiquitously in groundwater aquifer medium, and the cooperation effect was significant. Batch cultures results demonstrated that high nitrate, aniline, and COD removal efficiency (over 79.0, 96.6, and 69.6%) were achieved at selected C/N of 0.3, 0.46, and 0.91 conditions, and the maximum activities of HD and ANAMMOX reached to 25.22 mg/(d center dot l), 5.54 mg/(d center dot l), and 7.06 mg/(d center dot l) after 150 days inoculation. As such, nitrate, aniline, and COD removal efficiency were equal to 99.8, 99.9, and 89.5% in continuous flow column with nitrate 1,000 mg L-1 and aniline 50 mg L-1. The variations of