Effect of different bioremediation techniques on petroleum biomarkers and asphaltene fraction in oil-polluted sea water

摘要

The present study has clearly demonstrated that seeding a petroleum hydrocarbon polluted sea water with a halotolerant Pseudomonas aeruginosa Asph2 and corn steep liquor as a cheap and readily available source of nutrients significantly enhances the progress of biodegradation BD of petroleum hydrocarbon pollutants. There was a statistically high significant difference between natural weathering NW and both bioaugmentation BA (p = 2.92e-14) and biostimulation BS (p = 3.56e-13) at 95% confidence interval (alpha = 0.05). BA significantly improved the efficiency of total petroleum hydrocarbon TPH and the recalcitrant asphaltene degradation by approximate to 23 and 17% compared to BS process (p = 2.0693e-5 and p = 1.4e-3, respectively). The BD of biomarkers; pristane Pr, phytane Ph, and terpanes were very apparent. The BD of C28 tricyclic terpane, C27 13 beta, 17 alpha- diasterane (20S), and C30 17 alpha, 21 beta-hopane were higher than that of C35 17 alpha,21 beta-homohopane. The BD of C27 18 alpha(H)-22,29,30-trisnorneohopane (Ts) was higher than that of C27 17 alpha(H)-22,29,30-trisnorhopane (Tm). The BD of sterane was higher than that of hopane. C30 18 alpha-Oleanane was more resistant to degradation than other compounds.