Cytotoxic effect of current dental adhesive systems on immortalized odontoblast cell line MDPC-23

摘要

Objecitves; Evaluate the cytotoxic effect of the three dental adhesive systems. Methods: The immortalized mouse odontoblast cell line (MDPC-23) was plated (30, 000 cell/cm~2) in 24 well dishes, allowed to grow for 72 h, and counted under inverted light microscopy. Uncured fresh adhesives were added to culture medium to simulate effects of unset adhesive. Three adhesives systems were applied for 120 min to cells in six wells for each group: Group 1) Single Bond (3M), Group 2) Prime /100& Bond 2.1 (Dentsply), and Group 3) Syntac Sprint (Vivadent).