Unbound monomers do diffuse through the dentin barrier

摘要

Objectives. Assessing the role of dentinal fluid proteins in trans-dentinal diffusion of free monomers in vitro. Methods. An artificial pulp chamber (APC) topped human dentin disks was used. A simplified-two-step etch-and-rinse adhesive was formulated with 2-hydroethyl- methacrylate (HEMA), Bisphenol-A-diglycidyl-methacrylate (BisGMA), using Camphorquinone/tertiary amine as initiators. Two extraction media were used: buffered saline (Control), buffered saline with 1% bovine serum albumin (BSA). Samples were acid-etched, rinsed, air dried. Simplified primer was used, adhesive applied then light cured with a LED curing. Monomer diffusion was assessed by reverse phase HPLC.