The Influence of Salinomycin on the Expression Profile of mRNAs Encoding Selected Caspases and MiRNAs Regulating their Expression in Endo-metrial Cancer Cell Line

摘要

Background: Apoptosis could take place in the pathway dependent on death receptors orpathways dependent on mitochondria. In both, a key role is played by enzymes with protease activity,known as caspases.Aim: The aim of this study was to assess the variances in the expression pattern of caspase-dependentsignaling pathways in the endometrial cancer cell line when treated with salinomycin. Additionally, thechanges in the level of miRNA that potentially regulate these mRNAs were evaluated.Materials and Methods: Endometrial cancer cells were treated with 1 μM of salinomycin for 12, 24and 48 hours. Untreated cells made up the control culture. The molecular analysis consisted of screeningmRNA and miRNA microarray expression profiles of caspases, and the evaluation of the expressionof caspases 3,8 and 9 by RTqPCR, also on the protein level.Results and Discussion: It was observed that 5 of the 14 differentiating mRNAs were commonlyfound for all incubation times of the cells and they corresponded with CASP3, CASP8, and CASP9genes. The highest impact probability was determined between CASP3(up-regulated) and hsa- miR-30d (FC -2.01), CASP8 (down-regulated) and hsa-miR-21 (FC +1.39) and between CASP9 (upregulated)and hsa-miR-1271 (FC +1.71).Conclusion: Salinomycin induces the apoptosis of endometrial cancer cells. The largest increase inactivity was noted for caspases 3 and 9, while the expression of caspase 8 was decreased. Salinomycincauses a regulatory effect on the transcriptomes of mRNA and miRNA in in vitro endometrial cancercells.