Effects of Temperature on Germination, Emergence and Early Seedling Growth of Swards of Mt Barker Subterranean Clover Plants Grown with and without Nitrate

摘要

Effects of constant temperatures of 10, 15, 20, 25 and 30°C on the germination, emergence and early nvegetative growth of Trifolium subterraneum L. cv. Mt Barker grown as swards were examined in ntemperature-controlled glasshouses and in a growth cabinet. Seedlings were established at a density of nabout 2000 plants m-2 and grown for up to 70 days. Plants were either inoculated and grown without nmineral nitrogen (- N), or supplied with 7.5 mM NO; (+ N). nPercentage germination and emergence were hardly affected by temperatures of 10-2O0C, but at n25°C were reduced to 50'70, and at 30°C to about 10%. The rates of germination and emergence were nslowest at 1O0C, but showed little change with temperature over the range 15-30°C. Time to closed ncanopy (leaf area index 3) and time to a dry weight of 133 g m-2 were shorter where plants were supplied nwith NO; than where mineral nitrogen was withheld and a symbiotic system established. Rates of nN2-fixation, as measured by acetylene reduction assay, were not markedly affected by temperature over nthe range 10-25°C. Relative efficiency ranged from about 0.55 at 10, 15, and 20°C to about 0.66 at n25°C. At 30°C nodulation still occurred, but nitrogenase activity was very slight. nIt is concluded that, where swards of subterranean clover are grown in the absence of any mineral nN, a period of N-starvation limits growth during the time taken for symbiotic N,-fixation to become nestablished. Such retardation of growth is small at about 20°C, but becomes more marked at lower and nhigher temperatures. The establishment of subterranean clover swards in soils of low N status are likely nto be retarded following an early (March) or a late (July) start in the growing season. In such cases a n'starter' application of mineral nitrogen may promote the early growth of the legume.