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Identification and characterization of ten polymorphic microsatellite loci in the red panda Ailurus fulgens

机译:小熊猫小实蝇中十个多态微卫星基因座的鉴定与表征

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摘要

Ten polymorphic microsatellite loci were characterized from two genomic DNA-enriched libraries of the red panda (Ailurus fulgens). The number of observed alleles among 35 samples of red pandas ranged from five to 12. Observed and expected heterozygosities were 0.286–0.971 and 0.443–0.894, and the mean polymorphic information content was 0.712. All loci followed Hardy–Weinberg expectations except Aifu-14 and Aifu-16, which may due to the presence of inbreeding or null alleles. Three pairs of loci exhibited significant linkage disequilibrium after Bonferroni correction for multiple comparisons. These microsatellites would be useful to strengthen population management, genetic diversity exploration, and demographic history speculation of this species.
机译:从两个丰富的小熊猫基因组DNA的文库中鉴定出十个多态性微卫星基因座(Ailurus fulgens)。在35个小熊猫样本中观察到的等位基因数量在5到12之间。观察到的和预期的杂合度为0.286-0.971和0.443-0.894,平均多态性信息含量为0.712。除Aifu-14和Aifu-16外,所有基因座均遵循Hardy-Weinberg的预期,这可能是由于近交或无效等位基因的存在。 Bonferroni校正后,三对基因座表现出明显的连锁不平衡,可进行多次比较。这些微卫星将有助于加强该物种的种群管理,遗传多样性探索和人口历史推测。

著录项

  • 来源
    《Conservation Genetics》 |2008年第3期|787-790|共4页
  • 作者单位

    Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province Chengdu Research Base of Giant Panda Breeding Chengdu Sichuan 610081 China;

    Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province Chengdu Research Base of Giant Panda Breeding Chengdu Sichuan 610081 China;

    Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province Chengdu Research Base of Giant Panda Breeding Chengdu Sichuan 610081 China;

    Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province Chengdu Research Base of Giant Panda Breeding Chengdu Sichuan 610081 China;

    Key Laboratory for Reproduction and Conservation Genetics of Endangered Wildlife of Sichuan Province Chengdu Research Base of Giant Panda Breeding Chengdu Sichuan 610081 China;

    Nihon University 1866 Kameino Fujisawa Kanagawa 252-8510 Japan;

    Nihon University 1866 Kameino Fujisawa Kanagawa 252-8510 Japan;

    Nihon University 1866 Kameino Fujisawa Kanagawa 252-8510 Japan;

    Nihon University 1866 Kameino Fujisawa Kanagawa 252-8510 Japan;

    Nihon University 1866 Kameino Fujisawa Kanagawa 252-8510 Japan;

    Nihon University 1866 Kameino Fujisawa Kanagawa 252-8510 Japan;

    Nihon University 1866 Kameino Fujisawa Kanagawa 252-8510 Japan;

    Nihon University 1866 Kameino Fujisawa Kanagawa 252-8510 Japan;

    Nihon University 1866 Kameino Fujisawa Kanagawa 252-8510 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Red Panda; Ailurus fulgens; Microsatellite; (CA)n; (CAA)n;

    机译:小熊猫;小棕金;微卫星;(O)n(CAA)n​​;

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