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Development of 11 EST-SSRs for Japanese white birch, Betula platyphylla var. japonica and their transferability to related species

机译:为日本白桦Betula platyphylla var开发11种EST-SSR。粳稻及其与相关种的转移性

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摘要

Simple sequence repeat (SSR) markers were developed for Japanese white birch, Betula platyphylla var. japonica, using previously designed primer pairs derived from expressed sequence tags (ESTs). Out of 98 unpublished primer pairs, 35 yielded clear PCR amplification products, 11 of which revealed polymorphism in eight individuals sampled across the species’ range. The number of alleles detected and the expected heterozygosity ranged from 1 to 10 and 0.000 to 0.857, respectively, when these 11 loci were examined in 24 individuals from a single B. platyphylla var. japonica population. In cross-species transferability tests most of the 11 loci were also polymorphic in three other Betula species examined, but not B. maximowicziana. We have now developed a total of 25 polymorphic EST-SSRs for the genus Betula (including 14 we previously developed), which are likely to be highly useful in studies of various aspects of population genetics, including hybridization dynamics, in the genus.
机译:为日本白桦,白桦(Betula platyphylla var)开发了简单序列重复(SSR)标记。粳稻,使用先前设计的引物对,这些引物对来自表达的序列标签(EST)。在98个未公开的引物对中,有35个产生了清晰的PCR扩增产物,其中11个在整个物种范围内采样的8个个体中显示出多态性。当在来自单个白僵菌的24个个体中检查了这11个基因座时,检测到的等位基因数量和预期的杂合度分别为1至10和0.000至0.857。粳稻种群。在跨物种的可传递性测试中,在所检查的其他三个桦属物种中,11个基因座中的大多数也是多态性的,但不是最大双歧杆菌。现在,我们已经为桦属(Betula)属共开发了25个多态EST-SSR(包括我们先前开发的14个),这可能在该属中对种群遗传学各个方面(包括杂交动力学)的研究中非常有用。

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