Comparison Of High-sensitivity C-reactive Protein Serum Assayresults Obtained Using Dade-behring Bnii Nephelometer Andrnortho Vitros Fs 5.1 Clinical Analyzer In Respect Of Crp-related Riskrnassessment Of Chronic Metabolic Diseases

摘要

Background: Serum concentration of high sensitive C-reactive protein (hsCRP) can predict the risk of chronic metabolic and cardiovascular diseases but it is unclear whether turbidimetric high sensitive assays of CRP are adequate.rnMethods: Concentrations of serum CRP in 126 samples of serum were measured with high-sensitivity methods using nephelometry (BN II Nephelometer) and turbidimetry (Ortho Vitros FS 5.1).rnResults: For CRP concentrations measured by nephelometry and turbidimetry intra-assay CVs were 3.2 and 0.9% at mean CRP concentrations of 1.4 and 2.1 mg/l, inter-assay CVs for commercial controls were 3.1% and 3.6% at mean concentrations of 1.3 and 1.7 mg/1, and mean biases were 7.62% and 2.26%, respectively. Measurements were strongly, linearly correlated (r=0.99; CRP_(Vitros)=0.03 + 1.03CRP_(BNII)). When disease risk was assessed by nephelometry and turbidimetry, results were similar. If the risk of disease was classified as moderate (1.0 < CRP ≤ 3.0 mg/l) or high (CRP > 3.0 mg/l), the frequency of misclassified cases was only 2.3 and 2.1%, respectively. The classification agreement weighted k coefficient was 0.94 (95% C.I.: 0.89-0.98).rnConclusions: turbidimetric high sensitive CRP assays can properly classify CRP-related prediction of chronic metabolic diseases with special consideration on cardiovascular risk.