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机译:MIQE指南:发布定量实时PCR实验的最低信息
Stephen A. Bustin,1* Vladimir Benes,2 Jeremy A. Garson,3,4 Jan Hellemans,5 Jim Huggett,6 Mikael Kubista,7,8 Reinhold Mueller,9 Tania Nolan,10 Michael W. Pfaffl,11 Gregory L. Shipley,12 Jo Vandesompele,5 and Carl T. Wittwer13,141 Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and the London School of Medicine and Dentistry, London, UK, 2 Genomics Core Facility, EMBL Heidelberg, Heidelberg, Germany, 3 Centre for Virology, Department of Infection, University College London, London, UK, 4 Department of Virology, UCL Hospitals NHS Foundation Trust, London, UK, 5 Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium, 6 Centre for Infectious Diseases, University College London, London, UK, 7 TATAA Biocenter, Go teborg, Sweden, 8 Institute of Biotechnology AS CR, Prague, Czech Republic, 9 Sequenom, San Diego, California, USA, 10 Sigma-Aldrich, Haverhill, UK, 11 Physiology Weihenstephan, Technical University Munich, Freising, Germany, 12 Quantitative Genomics Core Laboratory, Department of Integrative Biology and Pharmacology, University of Texas Health Science Center, Houston, Texas, USA, 13 Department of Pathology, University of Utah, Salt Lake City, Utah, USA, 14 ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA.* Address correspondence to this author at: 3rd Floor Alexandra Wing, The Royal London Hospital, London E1 1BB, UK. Fax 44-(0)20-7377 7283, e-mail s.a.bustin@qmul.ac.uk.Received October 20, 2008, accepted January 27, 2009.Previously published online at DOI: 10.1373/clinchem.2008.112797Author Contributions: All authors confirmed they have contributed to the intellectual content of this paper and have met the following 3 requirements: (a) significant contributions to the conception and design, acquisition of data, or analysis and interpretation of data, (b) drafting or revising the article for intellectual content, and (c) final approval of the published article.Authors' Disclosures of Potential Conflicts of Interest: Upon manuscript submission, all authors completed the Disclosures of Potential Conflict of Interest form. Potential conflicts of interest:Employmentor Leadership: J. Hellemans, Biogazelle, J. Vandesompele, Biogazelle, C.T. Wittwer, Idaho Technology.Consultant or Advisory Role: R. Mueller, DermTech International.Stock Ownership: R. Mueller, Sequenom, C.T. Wittwer, Idaho Technology.Honoraria: None declared.Research Funding: S.A. Bustin, Bowel and Cancer Research, registered charity number 1119105, J. Hellemans, Fund for Scientific Research Flanders, M. Kubista, grant agency of the Academy of Sciences, Czech Republic (grants IAA500520809 and AV0250520701), C.T. Wittwer, ARUP Institute for Clinical and Experimental Pathology and Idaho Technology.Expert Testimony: None declared.Role of Sponsor: The funding organizations played no role in the design of study, choice of enrolled patients, review and interpretation of data, or preparation or approval of manuscript.,;
机译:MIQE指南:出版定量实时PCR实验的最少信息。
机译:MIQE指南:发布定量实时PCR实验的最低信息
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机译:使用基于免疫磁珠的回收率和实时免疫定量PCR(RT-iqPCR)分析快速,灵敏地检测动物饲料和粮食中的黄曲霉毒素。
机译:MIQE规范:实际实施基于荧光的定量实时PCR实验的最低标准指南
机译:mIQE指南:发布定量实时pCR实验的最低信息