Evaluation of the retention characteristics of ferrocene-bonded silica stationary phase and other silica-based reversed phases for HPLC using linear solvation energy relationships

摘要

The similarities and differences in retention characteristics of ferrocene-bonded and other three silica-based phases have been elucidated by the use of linear solvation energy relation-ships (LESRs). These phases were investigated in a common mobile phase. The results of LSERs on ferrocene-bonded phases are quite different from other silica-based phases. Retention on all other silica-based phases is dominated by two factors: the solute size and hydrogen bond acceptor basicity. While on ferrocene-bonded silica phase, the retention is dominated by excess molar refraction and hydrogen bond acceptor basicity. The s coefficient is small but positive which is unlike the common reversed phase stationary phases. Ther coefficient is much larger and positive showed the new phase has stronger π-π interaction to solutes than all other phases, and high selectivity to polyaromotic hydrocarbons.