Chromatographic Applications on Monolithic Columns: Determination of Triamcinolone, Prednisolone and Dexamethasone in Pharmaceutical Tablet Formulations Using a Solid Phase Extraction and a Monolithic Column

摘要

A method is described for extraction, separation and quantification of 3 corticosteroids in tablets (triamcinolone, prednisolone and dexamethasone). The extraction was achieved using a solid phase extraction (SPE) on RP-18 cartridges. The steroids eluates were examined by HPLC. The chromatographic separation was carried out on Chromolith® Performance RP-18e column, a new packing material consisting of monolithic rods of highly porous silica, using isocratic binary mobile phase of MeOH and H2O in the ratio 1: 1. A diode array detector was used at 254 nm for detection. The method was validated for system suitability, linearity, precision, limits of detection, specificity, stability and robustness. The limits of detection were 6.25, 6.25 and 12.50 ng per 25 μL injected volumes for them respectively. The recovery values of this method were between 89 and 101% and the reproducibility was within 6.86.