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>LC Determination of Helicidum in Beagle Dog Plasma: Study on the Pharmacokinetics of Helicidum Orally Disintegrating Tablets Compared to Conventional Tablets
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LC Determination of Helicidum in Beagle Dog Plasma: Study on the Pharmacokinetics of Helicidum Orally Disintegrating Tablets Compared to Conventional Tablets
A sensitive and reproducible liquid chromatographic method with UV detection was described for the determination of helicidum in beagle dog plasma. Sample preparation was accomplished through protein precipitation with 15% perchloric acid and chromatographic separation was performed on a reversed phase C18 column at 30 °C. Mobile phase consisted of a mixture of acetonitrile–water at a flow rate of 1.0 mL min−1. Wavelength was set at 270 nm. The method was applied to a pharmacokinetic study of two formulations of helicidum. No statistical difference in the t 1/2 (AUC0–5, AUC0–∞) between the two formulations were observed. The t max of helicidum ODTs is significantly shorter than that of conventional tablets (0.84 h vs. 1.33 h, P< 0.05).
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机译:描述了一种灵敏且可重复的紫外检测液相色谱方法,用于测定比格犬血浆中的螺旋藻。通过用15%高氯酸沉淀蛋白质来完成样品制备,并在30°C的反相C 18 sub>色谱柱上进行色谱分离。流动相由乙腈-水的混合物组成,流速为1.0 mL min -1 sup>。波长设定为270nm。该方法用于两种螺旋藻制剂的药代动力学研究。两种配方的t 1/2 sub>(AUC 0-5 sub>,AUC 0-∞ sub>)之间没有统计学差异。螺旋形ODT的t max sub>明显短于传统片剂(0.84 h vs. 1.33 h,P <0.05)。
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