Expression and cloning of ciliary neurotrophic factor gene from human optic glioma and glioblastoma cell line

摘要

Ciliary neurotrophic factor (CNTF) is the first neurokine to be found out for the potency to promote motoneuron survival in vitro and in vivo, and it does play a role in the development and regeneration of the nervous system. CNTF has been found up mainly in the astrocyte and Schwann cell. Its gene is located in the proximal part of long arm of chromosome 11, and apparently heterogenous to the gene of neurotrophins, there is no sequence for encoding signal peptide, We have demonstrated that during rat sciatic nerve regeneration, the gene expression of CNTF was downregulated, then it suggested that the expressed CNTF mRNA level would not be matchable to the degree of Schwann cell proliferation. The recombination of hCNTF has been reported. However, we tried another way, first, we tested whether it would express the CNTF gene in human optic glioma or human glioblastoma cell line by dot blot hybridization with rat CNTF cDNA probe, then cloned hCNTF cDNA from two kinds of cells by reverse transcription PCR. The final aim is to get the recombinant hCNTF.