Simple and effective detection method for gene expression product of transgenic plant

摘要

It is a crucial problem in gene engineering whether an exogenous gene could be cor- rectly expressed in cells of transgenic animal or plant, and how the expression products could be detected quantitatively in translation level. It is very difficult to analyze some gene expres- sion products, such as isopentenyl transferase(ipt), in transgenic investigation because they are trace in organisms. A convenient method is described to determine trace content gene ex- pression product which is hard to purify.